Journal: Bioelectronic Medicine

Article Title: Sympathetic innervation of human and porcine spleens: implications for between species variation in function

doi: 10.1186/s42234-022-00102-1

Figure Lengend Snippet: Comparison of different IHC antigen retrieval buffers and chromogens used to stain spleen tissue. Images were taken from three consecutive sections of spleen from a 57-year-old male (donor 004) and stained for TH. Images show white pulp (wp) and a central artery (ca.). A VIP purple chromogen using EDTA antigen retrieval showed greater intensity of nerve staining as well as higher degree of tissue degradation. B VIP purple chromogen using citrate buffer antigen retrieval better preserved the adventitia of blood vessels when compared to EDTA antigen retrieval. Additionally, spleen sections stained for TH using citrate antigen retrieval yielded less background staining than the other two methods. C ImmPRESS DAB chromogen using citrate buffer antigen retrieval showed fewer sympathetic nerves than adjacent sections

Article Snippet: To examine the association of TH + nerves with CD3 + T cells, double labeling experiments were performed using the Vector ImmPRESS Duet Double Staining Polymer Kit (Vector, MP7714), with a modified version of the manufacturer-recommended protocol.

Techniques: Staining